GENETIC DIVERSITY AND ANTIBIOTIC RESISTANCE PATTERNS OF PSEUDOMONAS AERUGINOSA ISOLATES FROM IRAQI HOSPITALS

H. F.   Naji; M. A.  AL-Jabber

doi:10.59807/jlsar.v5i1.93

Authors

H. F. Naji Department of Biology, College of Science, University of Babylon, Iraq.
https://orcid.org/0009-0007-1108-2098
M. A. AL-Jabber Department of Biology, College of Science, University of Babylon, Iraq.

https://doi.org/10.59807/jlsar.v5i1.93

Keywords:

P. aeruginosa, Multidrug-Resistance, DNA Sequencing, Virulence Factors

Abstract

Forty-six isolates of Pseudomonas aeruginosa were identified from 150 samples taken from different patients admitted to general hospitals in nine cities of Iraq during the period from September 2021 to January 2022. The isolates were named PA1 to PA46. The biofilm formation by these isolates was studied using microtiter plate assays, and the correlation between biofilm grades and antibiotic resistance was also analyzed. It was found that 34 isolates were able to produce biofilm. To determine the locations of blaTEM, blaCTX-M, and blaSHV genes on the genomes of the isolates, the whole genomic DNA of nine isolates, PA1 to PA9, was extracted, and the sequencing of these genes was achieved. Matching the isolates with NCBI GenBank global Pseudomonas strains showed that one isolate (PA1) was related to the UAE, two isolates (PA2 and PA3) were related to India, three isolates (PA4, PA5, and PA6) were related to Egypt, and three isolates (PA7, PA8, and PA9) were related to Iran. Hence, the variable frequencies in the sequencing of blaTEM, blaCTX-M, and blaSHV genes need further studies to create a genetic diversity map of P. aeruginosa.

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