EFFECT OF PLANT GROWTH REGULATORS ON IN VITRO AND EX VITRO CONDITIONS ON PROPAGATION OF DIANTHUS CARYOPHLLUS L.

Abstract

This study was carried out in the tissue culture Laboratory at University of Baghdad during 2017-2018 in order to evaluate the effect of plant growth regulators on the propagation of two cultivars of Dianthus (Sun charm White and Master) under Ex vitro conditions by cultivating the single nodes using the MS medium which involved with different concentrations of growth regulators The interaction treatment 1.0 mg /L BA + 0.2 mg /L NAA gave the highest response percent of bloom reached 100% for the red cultivar, and the treatment 1.5 mg / L BA + 0.2 mg / L gave the highest response percent to the white cultivar bloom reached 90%.In the multiplication stage, the highest branches number was obtained at 4.5 branches by the application of 2.0 mg / L BA + 0.2 and 0.4 mg /L IAA for the red cultivar, while the white variety gave the highest branches number obtained at the treatment 2.0 mg /L BA + 0.1 mg /L IAA, which gave 4.8 branches. In the experiment of rooting under Ex Vitro conditions, the results revealed that the concentration of 2 mg / L IBA gave the highest rooting ratio, number, and length of roots reaching 50%, 1.40 and 1.23 cm respectively for the red cultivar, while the white cultivar was given, 70%, 1.60 and 1.20 cm respectively, and the plants were adapted under greenhouse conditions.